As a former DC-3 and Beech 18 driver I can tell you two things. Unlike the Twin Beech, the tailwheel lock in a DC-3 is not all that critical. It is mainly beneficial during taxiing. But sure, it helps during takeoffs, especially heavily loaded when the tailwheel sticks the the ground a bit longer.
In a DC-3, also unlike certain other tail draggers, I would rather lose the rudder than ailerons. For directional control first you input rudder, simultaneously opposite aileron to rudder, and last but not least differential power. The ailerons are extremely effective for directional control, adverse yaw. DC-3 moves to the right, input right aileron. It is a given. It is a must. So, in the video, I don't see any aileron movement anywhere, not on the ground, not in the air...